產品名稱 ： NiNTA-μSpher
產品編號 ： P003
產品規格 ： 5mL
廠 牌 ： Biokit詳細資料鎳螯合層析填料,用於純化His-Tag蛋白
affinity purification matrix for His-Tag proteins. polymer uSphere-based chromatographic matrix with High Flow Rate. High capacity and high performance.NTA-mSphere is a nickel Affinity resin used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the mSphere ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.
IDA or NTA? Briefly, NTA chelates metal ion tighter that IDA and metal leakage low, but difficult to elute the target proteins. IDA chelates metal ion loosely and metal leakage is more but easier to elute the target proteins. Please see "NTA vs. IDA- a tale of two ligands" for details.
技術參數：
Particle Size (mm) Pore Size (Å) Ligand Density Target Protein Binding
Capacity (g/ml) Pressure
(MPa) pH Stability Suggested
Flow Rate（cm/h） Matrix
50 800 ~150mm Ni 5~90 mg of a His-tagged
protein MW 30 10 3~13 150-700 Polyacrylate
